Objective The goal of HeMiBio is to develop a hepatic microfluidic bioreactor from human iPSC-derived hepatocytes, hepatic sinusoidal endothelial cells (HSEC) and stellate cells (HSC), suitable for inclusion in a repeated dose toxicity testing strategy of pharmaceuticals/cosmetic ingredients. The successful creation of such a liver-device requires (a) homotypic and heterotypic interactions between the three cell types to induce and maintain their functional, differentiated state, and (b) optimisation of the matrix, oxygenation conditions, nutrient transport and physiological shear forces. The objectives are (1) to engineer the cellular components incorporated in the bioreactor to enable specific and spatially defined enrichment of the different cells from iPSC progeny, and, by gene editing, to allow non-invasive monitoring of the cellular state (differentiation and damage). (2) Aside from the molecular sensors, an array of electro-chemical sensors will be embedded in the reactors to assess liver-specific function and cellular health under repeated dose toxicity conditions, dynamically and in a high-throughput way. Cells and sensors will be built into (3) bioreactors that will be sequentially upgraded from 2D to 3D microfluidic reactors to ultimately allow full maintenance of mature functional hepatocytes, HSC and HSEC for >28 days. (4) As the ultimate goal is to use the device as a human-based alternative to rodent long-term hepatotoxicity studies, it will be of utmost importance to provide proof of concept that the 3D-devices reveal the hepatotoxicity of prototypical hepatotoxic compounds in vivo (5). “-Omics” and cell functionality studies will provide evidence that liver-like cells are present, exposed and affected by the selected toxic compounds. These ambitious objectives will be achieved by the excellent project team, composed of academic/industrial partners with unique and complementary biology, physiology, toxicology and technical skills from 7 EU Member States. Fields of science natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsengineering and technologyenvironmental biotechnologybioremediationbioreactorsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH.2010.4.2-9-2 - Exploitation of organ-simulating cellular devices as alternatives for long-term toxicity testing. FP7-HEALTH-2010-Alternative-Testing-Strategies Call for proposal FP7-HEALTH-2010-Alternative-Testing See other projects for this call Funding Scheme CP-IP - Large-scale integrating project Coordinator KATHOLIEKE UNIVERSITEIT LEUVEN Address Oude markt 13 3000 Leuven Belgium See on map Region Vlaams Gewest Prov. Vlaams-Brabant Arr. Leuven Activity type Higher or Secondary Education Establishments Administrative Contact Elke Lammertyn (Dr.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution € 788 019,20 Participants (12) Sort alphabetically Sort by EU Contribution Expand all Collapse all VRIJE UNIVERSITEIT BRUSSEL Belgium EU contribution € 585 899,65 Address Pleinlaan 2 1050 Bruxelles / brussel See on map Region Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad Activity type Higher or Secondary Education Establishments Administrative Contact Maria Vereeken (Ms.) Links Contact the organisation Opens in new window Website Opens in new window UNIVERSITETET I OSLO Norway EU contribution € 279 561,31 Address Problemveien 5-7 0313 Oslo See on map Region Norge Oslo og Viken Oslo Activity type Higher or Secondary Education Establishments Administrative Contact Eva Helene Mjelde (Ms.) Links Contact the organisation Opens in new window Website Opens in new window CONSORCI INSTITUT D'INVESTIGACIONS BIOMEDIQUES AUGUST PI I SUNYER Spain EU contribution € 276 801,80 Address Calle rossello 149 puerta bjs 08036 Barcelona See on map Region Este Cataluña Barcelona Activity type Other Administrative Contact Pastora Martinez Samper (Ms.) Links Contact the organisation Opens in new window Website Opens in new window THE HEBREW UNIVERSITY OF JERUSALEM Israel EU contribution € 480 144,40 Address Edmond j safra campus givat ram 91904 Jerusalem See on map Activity type Higher or Secondary Education Establishments Administrative Contact Jane Turner (Ms.) Links Contact the organisation Opens in new window Website Opens in new window INTERUNIVERSITAIR MICRO-ELECTRONICA CENTRUM Belgium EU contribution € 283 237,65 Address Kapeldreef 75 3001 Leuven See on map Region Vlaams Gewest Prov. Vlaams-Brabant Arr. Leuven Activity type Research Organisations Administrative Contact Christine Van Houtven (Ms.) Links Contact the organisation Opens in new window Website Opens in new window FRAUNHOFER GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG EV Germany EU contribution € 471 533,61 Address Hansastrasse 27c 80686 Munchen See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Andrea Zeumann (Ms.) Links Contact the organisation Opens in new window Website Opens in new window CSEM CENTRE SUISSE D'ELECTRONIQUE ET DE MICROTECHNIQUE SA - RECHERCHE ET DEVELOPPEMENT Switzerland EU contribution € 282 953,63 Address Rue jaquet droz 1 2000 Neuchatel See on map Region Schweiz/Suisse/Svizzera Espace Mittelland Neuchâtel Activity type Research Organisations Administrative Contact Olivier Guenat (Dr.) Links Contact the organisation Opens in new window Website Opens in new window MEDICYTE GMBH Participation ended Germany EU contribution € 279 578,83 Address Im neuenheimer feld 581 69120 Heidelberg See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Stefan Holder (Mr.) Links Contact the organisation Opens in new window Website Opens in new window MEDIZINISCHE HOCHSCHULE HANNOVER Participation ended Germany EU contribution € 87 768,62 Address Carl-neuberg-strasse 1 30625 Hannover See on map Region Niedersachsen Hannover Region Hannover Activity type Higher or Secondary Education Establishments Administrative Contact Frank Dittrich (Mr.) Links Contact the organisation Opens in new window Website Opens in new window UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET Norway EU contribution € 281 749,57 Address Hansine hansens veg 14 9019 Tromso See on map Region Norge Nord-Norge Troms og Finnmark Activity type Higher or Secondary Education Establishments Administrative Contact ørjan Olsen (Mr.) Links Contact the organisation Opens in new window Website Opens in new window INSERM TRANSFERT SA France EU contribution € 413 878,35 Address 10 rue d'oradour-sur-glane - parisante campus 75015 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Louis Jammayrac (Mr.) Links Contact the organisation Opens in new window Website Opens in new window UNIVERSITAETSKLINIKUM FREIBURG Germany EU contribution € 188 873,38 Address Hugstetter strasse 49 79106 Freiburg See on map Region Baden-Württemberg Freiburg Freiburg im Breisgau, Stadtkreis Activity type Higher or Secondary Education Establishments Administrative Contact Juergen Dreyer (Mr.) Links Contact the organisation Opens in new window Website Opens in new window
