Objective
Mammalian cells contain polymorphic ATP-dependent chromatin remodeling complexes similar to the yeast SWI/SNF complex. Because these complexes contain several subunits (including actin as well as DNA binding subunits) that are not present in the yeast complex the name BAF complexes have been adopted to raise a note of caution concerning mechanistic extrapolations. Recent work in the Crabtree laboratory has indicated that during neural development a series of these BAF chromatin remodeling complexes are expressed as cells differentiate from non-committed stem cells to periventricular neural stem cells to post mitotic neurons. For example, post mitotic neurons express the neural specific BAF53b protein, an Actin Related Protein (ARP) that occupies the place of BAF53a in the ubiquitous BAF complexes. Purification of the post mitotic neural-specific complex indicates that it contains at least 2 other subunits that are present only in neurons. Immunocytochemistry indicates that periventricular neural stem cells do not express BAF53a or b, suggesting that they have a specific complex. My goal will be to precisely define the components of the complexes present at different stages of neural development using the most recent and sensitive proteomic methods. I will be using mouse model system with different neuronal specific deletions of the BAF complex subunits such as Baf 53b and BrgI which have already been made in the Crabtree lab. If I confirm the substantial preliminary data that a neural specific complex is present, I will set out to understand why neural stem cells should have such a complex and weather or not it plays a role in the genomic plasticity associated with the stem cell state.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences medical biotechnology cells technologies stem cells
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-6
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
OIF - Marie Curie actions-Outgoing International Fellowships
Coordinator
AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.