Objective Inflammation, essential component of the innate immune response, allows multicellular organisms to repair damaged tissue. Excessive inflammation however, can itself exacerbate tissue damage, leading to chronic inflammation-related disease. The expression of crucial mediators of the inflammatory response is tightly regulated at transcriptional and post-transcriptional levels. Recent studies have shed some light on how cytoplasmic RNA processing events regulate mRNAs encoding inflammation mediators. However, it is unknown how nuclear post-transcriptional events control the expression of such molecules and what the contribution of RNA binding proteins (RBPs) in this control is.An RBP that has emerged as a pleiotropic modulator of mRNA function is HuR (Human antigen R) protein. In the cytoplasm, HuR stabilises many mRNAs via interplay with other RBPs. Research from the host laboratory has shown that over-expressed HuR represses translation of specific inflammatory mRNAs and attenuates acute inflammatory reactions, an unexpected role for HuR given its previous characterisation as an mRNA stabiliser. However, the role of HuR in the nucleus, where is most abundant, is still not understood. What is known is that nuclear HuR interacts, in an RNA dependent manner, with certain hnRNPs known to play a role in post-transcriptional regulation of gene expression. However, the role of these nuclear HuR-hnRNP interactions is unclear.The proposed research aims to understand the role of the nuclear HuR-hnRNP complexes in post-transcriptional regulation of inflammatory response in mouse macrophages. The Researcher’s expertise in studying ribonucleoprotein interactions in RNA function and processing in vitro will be combined with the Supervisor’s in functional study of post-transcriptional regulators in vivo and ex vivo. We will characterise the nuclear HuR-hnRNP complexes at both protein and RNA levels, and study their biological functions in regulating inflammatory gene expression. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicineimmunologynatural sciencesbiological sciencesgeneticsRNA Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2010-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Call for proposal FP7-PEOPLE-2010-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator BIOMEDICAL SCIENCES RESEARCH CENTER ALEXANDER FLEMING Address Fleming street 34 16672 Vari-athens Greece See on map Region Αττική Aττική Ανατολική Αττική Activity type Research Organisations Administrative Contact Babis Savakis (Prof.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution No data
