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Content archived on 2024-06-18

Structural studies of the interaction between Hsp70 and alpha-synuclein

Objective

"Protein misfolding and aggregation are associated with a wide range of severe, and so far incurable, neurological and systemic disorders, which include Alzheimer's and Parkinson's diseases, and type II diabetes. As these conditions are the consequence of the impairment of protein homeostasis, a variety of cellular natural defenses have evolved to eliminate protein aggregates as they form. In this application we are concerned with the problem of understanding the molecular basis of such defense mechanisms, by focusing on the specific case of the interaction between alpha-synuclein, a protein closely involved in Parkinson's disease, and Hsp70, a molecular chaperone with a variety of cellular functions, including the ability of recognising misfolded or aggregated proteins. We are going to address this problem using a wide arsenal of biophysical, biochemical and computational methods, including FRET and NMR spectroscopy, fluorescence methods, dynamic light scattering, transmission electron microscopy, isothermal titration calorimetry and molecular dynamics simulations. The outcome of this study will provide relevant structural information at atomic level on the binding modes of Hsp70 with diverse molecular forms of alpha-synuclein related to neurotoxicity. The results of this project are expected to increase our understanding of the molecular basis of the interactions between molecular chaperones and unstructured states of proteins, as well as to provide insights for the development of novel strategies for the rational design of therapeutic approaches against protein aggregation."

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2010-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
EU contribution
€ 209 592,80
Address
TRINITY LANE THE OLD SCHOOLS
CB2 1TN CAMBRIDGE
United Kingdom

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Region
East of England East Anglia Cambridgeshire CC
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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