Objective Genital herpes is the main cause of genital ulcers worldwide. Ulcerations resulting from recurrent HSV-2 infection consists one of the most common factors influencing integrity and functioning of genital mucosa and heighten the risk of HIV transmission. There are several types of cell death, of which apoptosis is a physiological process necessary for vaginal epithelium turnover during an oestrous cycle. Cell death receptors, such as Fas receptor together with its natural ligand, FasL, have been shown to participate in vaginal regression by inducing apoptosis. The Fas/FasL system plays an important role in skin and epithelial homeostasis, carcinogenesis and inflammatory skin diseases. Considering the fact that HSV-2 infection heightens the risk of other STDs, such as HIV and may involve exacerbation of apoptosis and inflammatory mechanisms, the idea of this project is to further explore the role of Fas/FasL system in regulation of the inflammatory and immune response and infection resolution during HSV-2 genital infection. The project will use the mouse model of genital herpes in the mice lacking Fas and FasL genes to compare inflammatory cytokines/chemokines production, local early immune response and efficiency of genital infection with the HSV-2 infection in wild-type mice. Further, the project will use the knowledge gained in an animal model to test how HSV-2 facilitates mucosal HIV-1 infection by eliciting inflammatory states in the in vitro human epithelial model. The will use up-to day techniques such as flow cytometry, DNA microarray assays, confocal microscope visualisation system and RNA intereference technology. The knowledge and expertise gained through this training project should help to designate and develop a new treatment methods (microbicides) in sexually transmitted diseases as well as to help a fellow to develop further independence as researcher. Fields of science medical and health sciencesbasic medicineimmunologyimmunisationmedical and health sciencesclinical medicinevenereal diseases Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Call for proposal FP7-PEOPLE-2010-RG See other projects for this call Funding Scheme MC-ERG - European Re-integration Grants (ERG) Coordinator SZKOLA GLOWNA GOSPODARSTWA WIEJSKIEGO Address Ul. nowoursynowska 166 02 787 Warszawa Poland See on map Region Makroregion województwo mazowieckie Warszawski stołeczny Miasto Warszawa Activity type Higher or Secondary Education Establishments Administrative Contact Marek Gabriel Niemialtowski (Prof.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution No data
