Objective
Nutrients overload leads to adipocyte hypertrophy and excess of adipose tissue causing deleterious consequences known as the metabolic syndrome. During my post-doc, we showed that the fat cell surface and the lipid droplet can communicate through an ER-ind ependent endocytic caveolar pathway of critical importance in targeting cholesterol to lipid droplets. This caveolin-dependent route could therefore function more generally to supply lipids to adipocyte lipid droplets. In order to identify key regulators o f this pathway, we will design a screen assay based on microscopy imaging. We will use caveolin fused to the green fluorescent protein (GFP) and the lipid droplet marker adipophilin fused to the red fluorescent protein (RFP). In lipid-loading conditions, c aveolins associate with fat bodies, resulting in the co-localization of the two proteins and yellow colour (merge of green and red emitted fluorescence). By combining this assay with RNAinterference of kinases genes, we hope to identify knock-downs which r everse this phenotype by screening for distinct red and green fluorescence patterns. An important issue is to compare these kinases requirements with the ones involved in caveosomes and clathrin-coated pathways.This project also aims at testing the physiol ogical importance of this caveolar-endocytic pathway in lipid droplet storage in vivo. Thus, we will identify lipid species able to stimulate association of caveolins with lipid bodies (starting from unstimulated conditions where caveolin and adipophilin d ont co-localize). We will in this case screen for induction of yellow colour. The physiological contribution of this pathway in nutrients intracellular storage will be evaluated by measuring concentrations of these lipids candidates in fat bodies isolated from animal models for metabolic syndrome. Identification of keys regulators of such a pathway can thus provide new perspectives for the control of lipid storage, a key feature in the battle against obesity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering colors
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-11
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.