Objective
Grapevine genetic transformation is a potential tool for both functional genomics and molecular breeding projects. Genomic resources of grapevine have been very little exploited and little is known on grapevine biology at the molecular level. Here we focus on the development of the methodology for efficient transformation of European cultivars to study gene function. There are three key points to approach grapevine transformation: The ability to produce plant tissue competent for transformation and regeneration, the choice of the quick method for transformation and regeneration and the design of DNA constructs for transgene integration. Anthers and ovaries will be used to initiate embryogenic cell suspensions, which will be assayed with novel cryopreservation techniques for keeping long-term regeneration capacity. We will select the biolistic device as strategy for transformation because of the advantage of the genegun for grape transformation avoiding the use of Agrobacterium, which causes hypersensitive reaction in grapes.
Whole circular plasmids will be compared to the novel minimal linear cassette technology for over-expression and silencing strategies of target grape genes. In this proposal, we will focus on grape genes involved in flower transition. We wi ll over-express VFL under control of the 35S-CaMV promoter as a possible strategy to reduce flowering time as well as the over-expression of the mutated VvGAI gene to obtain early flowering plants producing only inflorescences. We will approach the novel RNA interference (RNAi) technology as a tool for functional genomics studies by using the VAP1 gene, which silencing could affect tendril development. We expect to see early in vitro the effect of the expression strategy on the phenotype of recovered transgenic plants. The availability of an efficient grapevine transformation technology will allow approach in the future the study of genes involved in quality traits as well as approach molecular breeding programs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences developmental biology
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine embryology
- natural sciences biological sciences molecular biology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
SANTIAGO DE COMPOSTELA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.