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Microfluidic microdroplet femtoreactors

Objective

.We aim to develop a technology based on compartmentalising billions of experiments into separate microdroplets in microfluidic devices. These microdroplets, which can have a volume as small as ~5 femtolitres, are separated from other microdroplets by an immiscible phase. Identical microdroplets (<3% polydispersity) can be formed rapidly (at up to 104s-1), allowing ultra-high throughput (109 per day).

Furthermore, the microdroplets can be fused, allowing reagents to be mixed in a combinatorial manner, and reactions to be started, stopped or modified at defined times. Splitting microdroplets allows their contents to be aliquoted. If the reactions result in an optical signal, a spatially-resolved optical image of the microfluidic network will allow reaction kinetics to be determined. It will also be possible to separate microdroplets using a microfluidic flow sorter to allow recovery and further analysis or manipulation of the molecules they contain, notably by mass spectrometry.

Field of science

  • /natural sciences/chemical sciences/analytical chemistry/mass spectrometry
  • /natural sciences/physical sciences/classical mechanics/fluid mechanics/microfluidics

Call for proposal

FP6-2004-NEST-C-1
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Address
The Old Schools, Trinity Lane
Cambridge
United Kingdom

Participants (3)

UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6
France
Address
4,Place Jussieu
Paris
UNIVERSITÉ DE STRASBOURG
France
Address

Strasbourg
WEIZMANN INSTITUTE OF SCIENCE
Israel
Address
Herzel Street
Rehovot