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Content archived on 2024-05-29

Molecular Cloning and Functional Expression of Glyphosates Target Enzyme (EPSPS) in C3 turfgrass species


Glyphosate is a broad-spectrum (commonly referred to as non-selective) herbicide that is effective in inhibiting growth and controlling most plant species. The goal of the proposed research is to gain knowledge on the general processes contributing to plant competitiveness in agroecosystems that rely on herbicide tolerant crops.

The research links greenhouse based plant physiology with molecular marker techniques to understand the physiological and genetic basis for why certain species succeed in agroecosystems. The specific objective of this research is to characterize the variation in response of selected turf-grass populations to glyphosate on both the plant and molecular level. Plant process characterization will examine variation in glyphosate absorption, metabolism, and translocation.

Molecular characterization will include:
1) sequencing the EPSPS gene and deducing the amino acid sequence of the protein the gene encodes (this is the protein that glyphosate targets) and
2) determine gene copy number, and expression level.

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Faculty of Engineering and Natural Sciences Biological Science and Bioengineering Program Orhanli Tuzla

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