Objective
New antibiotics are urgently needed to combat persistent, emerging and re-emerging infectious diseases. Historically, microbial natural products have been a prolific source of antibacterials, and the screening of microbial extracts has yielded many classes of antibiotics. However, this approach was largely abandoned by big pharma because of the high rates of rediscovery of known antibiotics, and the general belief that there were very few new antibacterials to be discovered from microbial natural products.
Sequencing of bacterial genomes has changed that view completely. In many bacteria there are far more gene clusters directing the biosynthesis of antibiotic-like metabolites than natural products known to be produced by the organism. These so-called “cryptic” gene clusters (cryptic because their metabolic products are not known) are often silent (not expressed) under standard laboratory conditions, and can represent up to 80-90% of the secondary metabolite gene clusters in some bacteria.
In recent years we have witnessed an increased in the employment of cocultures (i.e. cocultivation of more than 1 microorganism) as a strategy to discover new natural products by exploiting cross-communication processes mediated by diffusible signalling molecules.
In this project we will carry out an in-depth study of the capacity of this strategy to discover new antibacterials from microbial metabolites. Objectives: a) Cocultivation of microorganisms from an actinomycete-based microbe collection employing a specifically designed dual fermentation system. b) Antibiosis screening of the obtained microbial extracts using modern high-throughput technologies; identification of active extracts c) LC-MS based chemical dereplication of active extracts using in-house and external natural product databases; identification of promising extracts d) Scale-up of active extracts, bioactivity-guided fractionation process to purify active compounds; structural elucidation of active compounds
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences microbiology bacteriology
- medical and health sciences health sciences infectious diseases
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs antibiotics
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-CIG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
18100 Armilla Granada
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.