Skip to main content
European Commission logo
English English
CORDIS - EU research results
CORDIS
CORDIS Web 30th anniversary CORDIS Web 30th anniversary
Content archived on 2024-05-27

Cytokinesis’ final cut: mechanics of abscission and ESCRT-III mediated membrane fission

Objective

"Abscission is the ultimate event of cytokinesis during which the intracellular bridge connecting two daughter cells is irreversibly cleaved. Reports show that a complex called ESCRT-III is involved in plasma membrane fission during abscission. The goal of this project is to understand how membrane mechanics can influence the fate of cytokinesis both at the molecular and at the cellular levels.

First, I will address the mechanism of ESCRT-III mediated membrane fission by using in vitro assays mimicking abscission geometry. I will study protein and lipid composition necessary for fission, as well as its dynamics and mechanical requirements by controlling membrane tension.

For the in vivo study, I will build a double pipette setup whose goal is to hold dividing cells, allowing to precisely visualize abscission in real time. Abscission mechanism will be addressed by controlling and measuring mechanical stress applied on cells in cytokinesis in combination with RNAi knock-down of ESCRT-III proteins.

By comparing in vivo and in vitro results, the ultimate goal will be to uncover the relation between abscission, ESCRT-III mediated membrane fission and cell mechanics."

Call for proposal

FP7-PEOPLE-2011-IEF
See other projects for this call

Coordinator

UNIVERSITE DE GENEVE
EU contribution
€ 184 709,40
Address
RUE DU GENERAL DUFOUR 24
1211 Geneve
Switzerland

See on map

Region
Schweiz/Suisse/Svizzera Région lémanique Genève
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Aurélien Roux (Dr.)
Links
Total cost
No data