Objective
Hematopoietic Stem Cell (HSC)-based therapies are becoming a great expectation for the cure of several disorders that require HSCs for treatment. However, the incapacity to generate and maintain fully potent transplantable HSCs in vitro is still nowadays one of the main limitations for their wide application in cellular therapies. The study of the molecular mechanisms underlying HSC ontogeny in embryos is thus of crucial importance in order to elucidate the genetic networks that control the emergence and expansion of these cells.
HSCs are first and possibly entirely formed in arterial-endothelium during embryonic life, a process dependent on Notch-signalling. However, the molecular events regulating these signals are mainly unknown.
Fringe-glycosyltransferases are Notch modifiers contributing to the differential response of Notch to distinct ligands. It is hypothesized that the cross-talk between Notch and its cognate ligands in the arterial endothelium compartment is primarily a consequence of Fringe-mediated modification and the driving-force towards endothelium-HSC transition.
This project aims the elucidation of the role Fringe as modulators of Notch-signalling towards specification of HSC identity at mid-gestation mouse embryos, an issue that has not been addressed so far. This work will be initiated with the identification of the Fringe involved on HSC emergence and with the study on how the modulation of their expression is correlated with Notch-ligand responsiveness and formation of hematopoietic precursors in vitro. The results obtained in this part of the project will be the basis of further in vivo experiments, using Fringe knock-in and knock-out animal models.
The execution of this proposal should provide relevant clues on important biological questions involving stemness and cell differentiation. Moreover, it will pave the way for the development of new and more robust strategies to produce and expand HSCs a crucial issue for regenerative medicine.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.