Objective
"Enzymes are essential for catalysis of the biochemical reactions that are characteristic of all living cells. Detailed explorations of these transformations have afforded a wealth of insight into the mechanisms of enzyme catalysis, heralding a range of enzyme applications. In spite of these advancements, further mechanistic studies on the fundamentals of enzymatic catalysis are still essential if we are to fully understand how enzymes effect the considerable 10e17 fold rate enhancements achieved under physiological conditions. A major challenge is to understand how the remote parts of the protein contribute to catalysis, that is; why are enzymes so big and how can mutations distant from the active site influence catalysis? Given that proteins are commonly built from only 20 amino acids, it seems reasonable a priori that during evolution of an enzyme, mutation of residues at remote sites have occurred to optimize the necessarily subtle changes in transition state configurations which must be achieved at the active site. Glycosidases are particularly appropriate model systems for such studies since they are ubiquitous, amenable to kinetic, structural and mutagenic studies and well organised into sequence defined glycoside hydrolase families. The xylanases from Cellulomonas fimi (Cex) and Bacillus circulans (Bcx) have been established as among the best mechanistically and structurally characterised enzymes, making them ideal systems with which to probe the roles played by remote residues in catalysis. In this project the fellow, Dr Martin A. Fascione will adopt a multidisciplinary approach to determine how subtle changes to TS configurations are transmitted from remote sites in enzymes using a combination of molecular biology, mutagenesis, protein semisynthesis, enzyme kinetics, protein NMR, all during the outgoing phase with Professor Stephen G. Withers (UBC), and protein X-ray crystallography during the reintegration phase with Professor Gideon J. Davies FRS (York)."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics mutation
- natural sciences chemical sciences catalysis
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IOF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
YO10 5DD York North Yorkshire
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.