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Identification of new targets of Wt1 during cardiovascular development

Objective

The epicardium is a mesothelial tissue that plays a crucial role during heart development, by contributing to the formation of cardiovascular precursor cells and by producing growth factors important for heart formation. Wt1 gene encodes a zinc finger protein, which plays a critical role in normal development of several organs such as kidney, gonads, spleen and heart. During heart formation, Wt1 is mainly expressed in the epicardium and epicardial derived cells. Deletion of Wt1 gene in an epicardial specific manner lead to a KO mouse with profound defect in both cardiomyocyte proliferation and coronary vascular development, suggesting that Wt1 KO epicardial cells constitute a good model to study the putative growth factors and signalling produced by epicardial cells. The principal objectives of the proposed project are to identify new targets of Wt1 during cardiovascular development, to explore their role in the formation of coronary blood vessels and cardiomyocyte proliferation and to dissect some of the mechanisms involved. We propose to study how Wt1 deletion, in vitro and in vivo, induced expression or repression of chemokines and growth factors and how this influences heart morphogenesis. The molecular mechanisms that regulate the development of the cardiovascular system in mouse embryos are comparable with those that regulate repair in adult tissue. The identification of new Wt1 targets during epicardium development, would allow us to design new strategies to activate those pathways during regeneration and repair of damaged hearts

Field of science

  • /natural sciences/chemical sciences/inorganic chemistry/inorganic compounds
  • /medical and health sciences/clinical medicine/embryology

Call for proposal

FP7-PEOPLE-2011-CIG
See other projects for this call

Funding Scheme

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

UNIVERSITAT DE BARCELONA
Address
Gran Via De Les Corts Catalanes 585
08007 Barcelona
Spain
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 100 000
Administrative Contact
Xavier Gutierrez (Mr.)