Objective
The discovery of type III secretion systems during the last decade has dramatically changed our understanding of the molecular basis of bacterial pathogenicity. Type III secretion systems are conserved in animal and plant bacterial pathogens and are involved in the delivery of bacterial effector proteins into the host cells. In plants, the identification of bacterial effectors is of an outstanding importance, since the understanding of the molecular processes leading to disease development and the identification of the corresponding molecular targets in the eukaryotic cell opens the way to new biotechnological strategies for crop protection.
In the plant pathogen R. solanacearum biochemical and genetic approaches led to the identification of several proteins that are exported through the Type III apparatus. The advent of the genome sequencing projects has facilitated the identification of new effectors based on the similarity or the structural features of predicted proteins, as well as the coregulation with th e Type III secretion system. A group of related genes that are called the awr family and have no similarities in the databases has been identified through analysis of R. solanacearum genome sequence. Mutation of one awr gene dramatically decreases R. solana cearum aggressivity on tomato, which renders the AWR proteins excellent candidates as effector proteins that carry out a function within the plant cell.
The aim of this project is to characterise thoroughly this gene family in search for type III-dependent effectors with a major role in pathogenicity. The process will include:
1) demonstration of protein translocation into plant cells,
2) study of their expression profile and analysis of the mutant phenotype regarding pathogenicity,
3) subcellular localisati on of the AWR effector proteins once inside the plant and
4) identification of the plant targets
5) establishment of the role of AWR effectors and their interactors in pathogenicity or defence.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant protection
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-11
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
BARCELONA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.