Final Activity Report Summary - DPP6 AND 10 PARTNERS (Investigate for cellular partners of DPP6 and DPP10 through novel technologies)
Our research is focussed on the elucidation of the physiological role of DPP6 and DPP10 through the identification of their binding partners and characterisation of their structure-function relationships. We are currently using an innovative and interdisciplinary approach, based on novel HTS (high throughput screening) selection of interaction partners, including recombinant antibodies targeting the different functional domains of DPP6 and DPP10. Our approaches includes the phage display, protein complementation assay and yeast two-hybrid technologies and led to the identification of some proteins of the cytoskeleton as putative binding partners of DPP10 and the development of three isoform-specific recombinant antibodies targeting the intracellular domain of DPP6.
These data will add impetus to the development of inhibitor molecules for pharmacological or therapeutic use.