Objective
The nuclear pore complex (NPC) mediates all traffic between the nucleus and cytoplasm and serves as a central node of cellular communication. Despite the recent identification of the full complement of nuclear pore proteins, much of the molecular details of NPC structure and its mechanism of action, remain unknown. Moreover, the precise molecular pathway for NPC biogenesis is largely undefined. Here, I present a comprehensive approach for dissecting the mechanism of NPC assembly, using in vitro nuclear reconstitution in Xenopus egg extracts. My working hypothesis is that NPC assembly begins with the formation of fused double nuclear membranes. In this model, an initial fusion event between the inner and outer nuclear membranes triggers the assembly process, and soluble nucleoporins are then added in a sequential manner, to form the complete structure.
A prediction of this model is that pore-membrane protein(s) should play a vital role at the early stages of assembly. To test this prediction we will use morphological analysis and immunolocalization of the pore-membrane proteins by transmission electron microscopy. Aborted assembly intermediates will be isolated and used in combination with fractionated cytosol to identify subsequent steps in the process. Newly identified assembly intermediates will be characterize by fluorescence and electron microscopy. The overall goal is to reach a detailed step-by-step model of NPC assembly. This will promote our understanding of the biogenesis and function of one of the largest supramolecular assemblies in eukaryotic cells, and the gateway to the genome.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine medicinal chemistry
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
HAIFA
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.