Objective
In the immune response, a resistance protein from the host (R) recognizes an avirulent protein from the pathogen (Avr) and elicits a defence response that abrogates the infection attempt. The immune response is generally accompanied by the hypersensitive response (HR), a form of programmed cell death (PCD) that manifests within hours after R/Avr recognition. In a random Virus-Induced-Gene-Silencing (VIGS) screening of a Nicotiana benthamiana cDNA library, I identified several genes that participate in the HR. Among those genes, I characterized in detail a MAPK kinase kinase kinase (MAPKKKa), a positive regulator of cell death in inmunity and susceptibility. I also identified a calcineurin B-like protein (NbCBL) and its target, a CBL-interacting protein kinase (NbCIPK).
These results implicate for the first time members of the CBL and CIPK families in biotic stress signalling. As a short-term goal, this project will focus on the characterization of NbCBL and NbCIPK in the immune and susceptible responses to pathogens in Solanaceae and Arabidopsis. For this purpose, I will use functional, biochemical, sub-cellular localization and genetic approaches. I will also investigate if there is a connection between the phosphorylation cascades elicited by CBL-CIPK and MA PKKKa. As a mid-long term goal, I plan to establish an optimised Forward Functional Genomics Screening strategy aimed to the targeted identification of genes that participate in early signalling events in the immune response (between R/Avr and MAPKKKa).
Fields of science (EuroSciVoc)
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
SEVILLA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.