Objective
Seed biology is crucial to improve the nutritional value of agronomically important seeds. While seed development has been extensively studied, very little is known about the process by which the embryo emerges from the seed to complete germination. This project is aimed to a better understanding of the molecular mechanisms that control germination by identifying and characterizing key regulatory genes and conserved promoter cis-elements. Data from global gene expression analysis (micro-arrays) in the model plant Arabidopsis thaliana will be analysed to identify genes induced specifically upon germination and putatively involved in different key aspects of germination. For selected genes we will isolate the promoters of their orthologous genes in other plant species from within and outside the Brasicaceae family.
We will compare those promoters and perform in silico analysis to identify conserved motifs, which will be strong candidates to be functionally important cis-sequences. Identified sequences will be characterized in vivo and those being transcriptionally active will be used in a yeast one-hybrid screening with a normalized expression library containing approx. 75% of Arabidopsis transcription factors (TFs). The identified TFs will be subjected to functional analysis using reverse genetic approaches. The results from this research will abound in the complex mechanisms underlying seed germination and possibly in other intimately linked seed processes such as seed development and dormancy. The knowledge generated may benefit the crop industry by helping enhance plant fitness and by providing valuable molecular markers to use in marker- assisted breeding.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences biological sciences genetics RNA transcriptomes
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- agricultural sciences agriculture, forestry, and fisheries agriculture
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.