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Development of an "anti-disease" vaccine and diagnostic tests for African trypanosomosis

Objective

The aim is to contribute to improvement of livestock productivity in DC through limitation of trypanosome-associated pathology and accurate diagnostics of trypanosome infections. It is proposed to develop immunisation strategies against pathogenic factors of trypanosomes (anti-disease vaccine). In a previous INCO project we focused on congopain, an immunosuppressive cysteine protease (CP) of Trypanosoma congolense. As the effects of immunisation with congopain are limited, association with other antigens is required. The current proposals expand the initial work to screening and characterisation of other pathogenic molecules, especially those responsible for anaemia. A number of other trypanosome proteases - CPs, serine and metallo- proteases - require characterisation as to their biological roles in the parasite and the host. Trypanosomes also contain protease inhibitors, some of which have immunomodulatory effects. They may thus be manipulated, directly or in association with their partner enzymes, to modulate the disease. Other, non-proteolytic, factors have been identified. The GPI (glycosyl phosphatidyl inositol) anchor of the T. brucei Variant Surface Glycoprotein is a major inducer of TNF, a cytokine that mediates anaemia. Finally, recent development in the field of proteomics and progress in the genome mapping of trypanosomes provide tools to study new pathogenic pathways. In order to improve diagnosis of the disease, procedures for antibody detection will be developed and/or validated. They are based on recombinant technology, which circumvent problems associated with the current use of parasite extracts. Recombinant and synthetic peptides from CPs and heat shock proteins, both previously identified as major antigens, as well as newly described molecules, will be assessed for their diagnostic potential. Techniques for detection of parasite antigens in the host tissues will be re-examined using recently developed monoclonal antibodies.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/genome
  • /social sciences/economics and business/economics/production economics/productivity
  • /medical and health sciences/basic medicine/pathology
  • /medical and health sciences/health sciences/public and environmental health/epidemics prevention/immunisation
  • /natural sciences/biological sciences/microbiology/protozoology
  • /medical and health sciences/basic medicine/pharmacology and pharmacy/pharmaceutical drug/vaccines
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/proteomics
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /natural sciences/biological sciences/zoology/mammalogy

Call for proposal

FP6-2002-INCO-DEV-1
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

CENTRE DE COOPÉRATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LE DÉVELOPPEMENT
Address
42, Rue Scheffer
Paris
France

Participants (11)

CENTRE INTERNATIONAL DE RECHERCHE-DÉVELOPPEMENT SUR L'ÉLEVAGE EN ZONE SUBHUMIDE
Burkina Faso
Address
559 Rue 5 Et 31 Ave Du Gouverneur Louveau
Bobo Dioulasso
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
3, Rue Michel-ange
Paris
DIAMED AG
Switzerland
Address
Pra Rond
Cressier S/morat
FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW
Belgium
Address
Rijvisschestraat 120
Ghent
MAKERERE UNIVERSITY
Uganda
Address
Makerere Hill Road
Kampala
PRINS LEOPOLD INSTITUUT VOOR TROPISCHE GENEESKUNDE
Belgium
Address
Nationalestraat 155
Antwerpen
UNIVERSIDAD SIMÓN BOLÍVAR
Venezuela
Address
Sartenejas
Caracas
UNIVERSIDADE EDUARDO MONDLANE FACULDADE DE VETERINARIA
Mozambique
Address
Praça 25 De Junho
Maputo
UNIVERSIDADE NOVA DE LISBOA - INSTITUTO DE HIGIENE E MEDICINA TROPICAL
Portugal
Address
Rua Da Junqueira, 96
Lisboa
UNIVERSITY OF GLASGOW
United Kingdom
Address
University Avenue
Glasgow
UNIVERSITY OF KWAZULU-NATAL
South Africa
Address
University Road, Chiltern Hill
Westville