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CRYo-banking of Stem cells for human therapeutic application

Objective

Stem cells are at the centre of biomedical research. The development of human stem cell therapies will have to build on a safe and reliable supply of human stem cells which must be assured by cell banking. Currently, the isolation, identification and culture of stem cells are not standardised between laboratories, and reproducibility of protocols is limited. Today's banking approaches still rely on storing sources of stem cells rather than on banking of defined, well-characterised stem cell populations (both adult and embryonic). Cryopreservation of stem cells itself is not yet optimised and validated for the different cell types, and multiple cell biological and biophysical challenges remain to be addressed in order to define optimised cryopreservation protocols.
The problems currently limiting the routine application of stem cell banking with a therapeutic perspective will be addressed by the CRYSTAL project. The consortium will develop tools and optimised procedures to enable cryopreservation of different stem cell types and allow safe production of sufficient numbers of high-quality cells for future human therapy. This will comprise standardised protocols and tools for stem cell isolation, identification and culture, novel approaches to their cryopreservation (e.g. novel cryoprotectants, freezing in different conformations) and an automated quality control system for stem cell preparations.
Five stem cell research laboratories providing four different sources of adult (from cord blood, bone marrow and placenta) and human embryonic stem cells have teamed with two partners specialising in applied banking and fundamental cryobiological research. CRYSTAL is thus in a position to solve existing problems in an integrated, systematic approach and to provide standardised, reproducible methods and tools to advance therapeutic stem cell research in Europe.'

Call for proposal

FP6-2005-LIFESCIHEALTH-6
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

UNIVERSITY OF COLOGNE /CLINICAL CENTRE OF THE UNIVERSITY OF COLOGNE
Address
Kerpener Str. 62
Cologne
Germany

Participants (8)

CRYO-SAVE GROUP NV
Netherlands
Address
Ijsselkade 8
Zutphen
MEDICAL UNIVERSITY OF VIENNA
Austria
Address
Spitalgasse 23
Vienna
KATHOLIEKE UNIVERSITEIT LEUVEN
Belgium
Address
Oude Markt 13
Leuven
UNIVERSITY OF ANTWERP
Belgium
Address
Prinsstraat 13
Antwerpen
ARTTIC
France
Address
58 A Rue Du Dessous Des Berges
Paris
TISSUEGNOSTICS GMBH
Austria
Address
Taborstrasse 10/2/8
Wien
FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Germany
Address
Hansastraße 27C
München
UNIVERSITÄT ZÜRICH
Switzerland
Address
Rämistr. 71
Zurich