Skip to main content

Capture and enrichment of emerging pathogens for multiple and ultra-sensitive diagnostic

Objective

In the 1970's WHO proclaimed that eradication of smallpox should be attempted. This goal has been achieved 1979. There is now consensus that list of newly emerging or re-emerging pathogens is continuously growing. During the last decades pathogens such as Marburg, Ebola, Hepatitis-C and Hantavirus, HIV and more recently, SARS coronavirus have emerged. Today the risk of a new influenza pandemic again highlights the global threat of infectious diseases. In addition, the possibility of bioterrorist attacks using highly pathogenic viruses and bacteria can not be ignored. Furthermore, the requirements for sensitivity and specifity of diagnostics have increased. A major obstacle for the detection of pathogens in clinical or environmental samples are false negative results, e.g. for HCV "occult infections". This is mainly due to the lack of a rapid and reliable pathogen concentration methodology, and the inability of most of the currently used technologies to eliminate or neutralize interfering "natural inhibitors" present in samples. The aim of this research program is to exploit the "non-self" recognition and binding properties of human apolipoprotein H (ApoH). ApoH binds and captures pathogens enabling their concentration from different biological samples. Magnetic beads coated with ApoH recombinant protein can be efficiently used as a pretreatment step to greatly improve the detection threshold and thereby increasing the sensitivity for diagnosis of emerging pathogens, regardless of the molecular techniques used for their diagnosis. The project will focus on viruses and bacteria from clinical and environmental samples including those that could be used in bioterrorism. In addition to the incorporation of ApoH into regular molecular techniques for pathogen detection, mini-array chips will be developed for multiple and ultra-sensitive pathogen diagnosis.'

Field of science

  • /medical and health sciences/health sciences/infectious disease/RNA virus/coronavirus

Call for proposal

FP6-2005-LIFESCIHEALTH-7
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

ROBERT KOCH-INSTITUT
Address
Nordufer 20
Berlin
Germany

Participants (8)

IMMUNOCLIN LIMITED
United Kingdom
Address
Rowlandson House 289 To 293 Ballards Lane
London
APOH-TECHNOLOGIES
France
Address
94 Allée Des Fauvettes
La Grande Motte
PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE
Chile
Address
Avenida Del Libertador Bernardo O'higgins 340
Santiago
EBIOCHIP SYSTEMS GMBH
Germany
Address
Fraunhoferstr.1
Itzehoe
SARL SKULD-TECH
France
Address
Rue Du Curat, 134
Montpellier
FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Germany
Address
Hansastrasse 27 C
München
GENEXPRESS GESELLSCHAFT FÜR PROTEINDESIGN GMBH
Germany
Address
Eresburgstr. 22-23
Berlin
INSTITUT DE RECHERCHES POUR LE DÉVELOPPEMENT
France
Address
44, Bd De Dunkerke, Cs 90009
Marseille