Objective
We propose to physically trap a single bio-molecule into successive conformational sub-states by rapidly freezing and thawing it with its immediate surroundings. After many successive freeze-thaw cycles, we will reconstruct the dynamics of the same single molecule from the series of frozen snapshots, probed by the fluorescence of optical labels. Fast cooling and heating will be achieved by maintaining the molecule in a focused laser spot within a cold cryostat. We will control the local temperature by modulating the power of the heating beam. The time response, given by heat diffusion out of the diffraction limited hot spot is as short as a few microseconds. In the past several years unique know-how and infrastructure have been developed and feasibility of quick freeze-thawing cycles has been demonstrated in Huygens Laboratory, Leiden University. In the coming years, we want to extend the measurements first to oligopeptides and small proteins, then to larger proteins in physiological conditions. We expect this project to open a completely new window on protein dynamics, by enabling us to observe the same single molecule over large time intervals, at high-time resolution. The series of snapshots will give information on relaxation processes down to microsecond timescales, inaccessible to other single-molecule methods, and make it possible to map out the topography of the energy landscape, i.e. not only the accessible conformational sub-states, but also the favored pathways connecting them.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences astronomy planetary sciences planetary geology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics laser physics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-7
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
LEIDEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.