Objective
Iron is an essential element for plant metabolism. Coordinated at the active site of various metalloproteins, iron is involved in many enzymatic reactions such as photosynthesis, respiration, nitrogen fixation, DNA and hormone synthesis. In addition, plants have to face great differences in iron availability in the environment because of their immobility, and either starvation or excess of this element can be responsible for severe nutritional disorders.
A preliminary survey by WARDA (West Africa Rice Development Association) in 2001 suggested that as much as 60% of the low land rice area in West and Central Africa may be at risk from iron toxicity leading to yield losses varying from 12% to 100%. Previous work at Montpellier has shown that several proteasome proteins respond to iron overload and that the ferritin gene (AtFer1) induction triggered by iron can be abolished by proteasome inhibitors such as MG132. Moreover, a burst of nitric oxide occurs in the chloroplast after an iron overload.
These data suggest that ubiquitination (possibly SUMOylation) and nitrosylation of proteins are part of the iron overload signalling pathway. To identify, in the plant model Arabidopsis thaliana, genes involved in the signalling of iron toxicity we will combine modern proteomics and bioinformatics tools to classical molecular genetics approaches. For this purpose, we will use the promoter of the ferritin-encoding gene AtFer1 as the terminal target of this transduction pathway and develop two innovative and complementary proteomics approaches.
As focusing at the protein and sub-cellular levels, we will analyse S-nitrosylated proteins in the chloroplasts and ubiquitinated/SUMOylated proteins in the cytoplasm/nucleus. This combination should bring novel insights on crucial post -translational features that were largely overlooked yet. In addition, the tools we propose to develop within this frame are likely to be useful for analysing other responses in plants including crops.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
- natural sciences biological sciences botany
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.