Objective
Proteins interact with genomic DNA to bring the genome to life. The precise regulation of numerous genes is required to maintain normal cellular proliferation and differentiation. A key step in the regulation of networks that control gene expression is the sequence-specific binding of transcription factors to their DNA recognition sites, and sequence-specific DNA binding by proteins even controls recombination, restriction, and replication. Understanding where regulatory proteins bind to the genome in vivo is critical to understanding the mechanism and logic of these essential processes. Available methods to determine sequence requirements suffer from either insufficient specificity, sensitivity or a limited potential for multiplexed analysis.
Therefore, I w ill develop a highly parallel and sensitive method for studying the sequence specificity of DNA-protein interactions. My approach will be based on the sensitive proximity ligation assay developed in my host laboratory and will include a multiplexed readout using tag arrays. This technique will allow functional analysis of DNA binding proteins at an unprecedented scale and with the potential to analyse even individual cells, providing a unique perspective on gene regulation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences health sciences infectious diseases RNA viruses influenza
- medical and health sciences clinical medicine oncology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
UPPSALA
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.