Objective
The current research proposal claims to carry out the technology of the enzymatic bioremediation of polycyclic aromatic hydrocarbons (PAHs) by using biocatalysts (both native and tailored by directed molecular evolution). PAHs are a class of highly dangerous xenobiotics widely distributed in terrestrial and aquatic environments.
Last trends in PAHs removal address to combine chemical and biological approaches for remediation of these persistent contaminants. However, high molecular weight PAHs cannot be successfully metabolized by neither autochthonous populations nor genetically manipulated bacteria, both under in situ and ex situ applications. In this context, the only organisms known to oxidize efficiently high molecular weight PAHs are the white rot fungi.
Many shortcomings have hindered the application of such organisms in large-scale bioremediation processes. Laccase is the one of the most promising and versatile biocatalyst in PAHs oxidation. This proposal deals with the use of laccases in PAHs detoxification from sea spills and wastewaters. The dependence of redox mediators and the instability of laccases are the main hurdles for a practical application.
The study of laccases in PAHs detoxification will be tackled by:
- Biocatalyst engineering by directed molecular evolution to improve the enzyme stability against organic solvents.
- The enhancing the redox potencial of lacccases will mean the increasing in the PAHs oxidation. The construction of combinatorial libraries by saturation mutagenesis will be performed to enhance the laccase ionization potential.
- The biocatalyst will be immobilized on acrylic supports and used in a fixed-bed bioreactor to be tested with waste-waters contaminated with PAHs.
The objectives of this Project are relevant to two of the activity areas of the Sixth Framework Program:
- Life sciences, genomics and biotechnology for health;
- Sustainable development, global change and ecosystems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences chemical sciences organic chemistry hydrocarbons
- natural sciences chemical sciences catalysis biocatalysis
- natural sciences biological sciences molecular biology molecular evolution
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-7
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.