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Content archived on 2024-05-29

Site-specific labelling of proteins for Fluorescent Tagging or Immobilisation using 'Click'-Chemistry or Staudinger Ligation

Objective

Yong-Qing Yang is a highly talented young chemist in the final year of her PhD at Shanghai Institute of Organic Chemistry (SIOC), Chinese Acad. Sciences, a world-leading Science & Technology Institution.

She has already demonstrated significant potential to be a leading researcher through the important work that she has published in leading International chemistry journals. Her expertise in synthesis provides her with a strong platform of practical skills to complement those in chemical biology of Dr Thomas- group at Nottingham Univ. in the proposed multidisciplinary research.

This project aims to develop tools to selectively modify ONLY a single protein in a cell with a functionalised biotin, and then to use this versatile biotin 'handle' to conduct further 'bio-orthogonal' chemistry (Staudinger ligation, 'click-chem') allowing the introduction of fluorescent, crosslinking or immobilisation tags ONLY onto the biotin analog. Using these tags transient protein-protein interactions and protein location in-vivo can be identified.

This will provide new fundamental proteomics information forming the basis for drug discovery as fits several FP6 objectives including 'Application of knowledge and technologies in the field of genomics and biotechnology for health: rational and accelerated development of new, safer, more effective drugs'. Training at Nottingham will involve Ms Yang obtaining new practical skills in biophysical and biochemical techniques.

She will then be in a strong position to act as a leader and female role model in interdisciplinary 'Chemical Biology' research in China. She will participate in the Nottingham Career Advancement program that covers managerial, IP protection and leadership skills courses for young researchers.

The biotin tools developed in Nottingham will be transferred back to China, and, through collaboration applied to identify the effect of traditional Chinese medicines at a molecular level using Western Medicinal Chemistry techniques.

Call for proposal

FP6-2005-MOBILITY-7
See other projects for this call

Coordinator

THE UNIVERSITY OF NOTTINGHAM
EU contribution
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Address
Centre for Biomolecular SciencesUniversity Park
NOTTINGHAM
United Kingdom

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