Objective
The study of tolerance mechanisms of higher plants against abiotic stress conditions to improve the production of crop plants is a very important research area in the world. In this project we will use yeast to identify genes of Selaginella lepidophylla th at are important for its exceptional drought tolerance.
This plant is able to survive in a complete dry form for many years. One of the reasons for its high stress tolerance is the very high trehalose levels found in this plant. Trehalose is well-known for its stress protection characteristics. It is synthesised in two steps. Tps1 catalyzes the formation of trehalose-6-phosphate, which is then converted to trehalose by the activity of the Tps2 enzyme.
Previously we have isolated the S. lepidophylla TPS1 gene . Expression of this gene in a yeast tps1 mutant did not complemented the deletion phenotype (growth on glucose and trehalose synthesis). N-terminal truncation of this gene, however, resulted in an allele with very high activity upon expression in yeast, indicating N-terminal inhibition on the activity of the rest of the protein.
We have constructed a S. lepidophylla cDNA library in a yeast expression vector and we will use this library to screen for genes that may interact with the N-terminal sequence of the previously characterized SlTPS1 gene by selection of transformants that grow on glucose and that produce high levels of trehalose. So far, no real TPS2-like genes have been isolated from plants.
As S. lepidophylla accumulates high levels of trehalose, we expect a functional TPS2 enzyme to be present. We will use the library to transform the temperature sensitive yeast tps2 strain and screen for transformants that allow growth at 39 °C.
Finally we will also identify other components that may increase stress tolerance of yeast cells. The components resulting in the best stress tolerance in yeast cells will also be transformed into A. thaliana and. tested for stress tolerance.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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- agricultural sciences agriculture, forestry, and fisheries agriculture
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences botany
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-7
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IIF - Marie Curie actions-Incoming International Fellowships
Coordinator
ZWIJNAARDE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.