Objective
The European Community produces every year 200 million tonnes of biodegradable wastes, which have to be diverted from land-filling according to European laws. Biological digestion, either aerobic or anaerobic, is the most environmentally favourable option f or the recycling of biowastes. Composts resulting from these processes are used as soil improvers, which, among other benefits, exert plant disease supressiveness due to the presence of microorganisms that are antagonists to pathogens. However, improperly treated composts may spread pathogenic microorganisms, thus damaging the environment and posing risks to human health. This causes concern among the general public, especially after the food alerts during the last decade, and constitutes a priority issue within the European Research Area.
Therefore, a tool that allows the quick analysis of compost microbiota is needed to safeguarde its agronomic value. DNA biochip technology offers the possibility to simultaneously detect an entire array of micro-organisms. The host group has recently designed a micro-array addressed towards compost microbiota. This proposal aims to design an oligonucleotide micro-array to detect signature microorganisms in composts from anaerobic digestion. Micro-organisms that will be isolated from compost will be targeted. Additional probes will be selected on basis of sequences determined from bands in DGGE gels run with compost DNA. Oligonucleotide probes will be designed using sequence differences in the 16S rDNA gene and printed onto the arrays.
Fluorescently labelled DNA will be prepared and hybridised, and the arrays analysed by fluorescent screening. The validation of the micro-array will be mainly conducted through spiking experiments and through their wide application to already characterised compost samples. If successful, the micro-array might permit assessing whether soil improvers meet hygiene demands, thus increasing their marketability and promoting confidence among European consumers
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology environmental biotechnology bioremediation bioreactors
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- engineering and technology environmental biotechnology bioremediation compost
- natural sciences chemical sciences organic chemistry aliphatic compounds
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
INNSBRUCK
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.