Objective
Sodium/hydrogen exchangers (NHEs) are ion transporters catalysing the electroneutral exchange of sodium with protons in prokaryotes and eukaryotes. NHE1 is a ubiquitous mammalian plasma membrane transporter. In addition to the control of cell volume and cell pH, NHE1 regulates cell migration and mechanosensation, which in turn contribute significantly to the pathogenesis of cancer and cardiovascular diseases in humans.
Since the underlying molecular mechanisms of this regulation remain elusive, I have made use of a novel generation of yeast-two-hybrid assays and identified several putative regulators of NHE1. The aim of this proposal is to analyse these newly identified NHE1 interacting proteins in the context of NHE1 regulation with emphasis on cell migration and mechanosensation.
For this purpose, I will be combining biochemical and imaging studies for a detailed analysis of these protein interactions with the help of recently established fibroblast cell lines I created from NHE1 wild-type and knock-out mice. In-depth analysis on the impact on NHE1 activity regulation will be done by our new assay combining self-referencing ion-selective microelectrodes during whole-cell patch-clamp with concomitant pipette perfusion. Boyden chamber and wound healing scratch assays will be used to detect how NHE1 interacting proteins affect cell migration.
This multi-disciplinary approach will uncover molecular mechanisms of NHE1 regulation and inevitably shed light on how NHE1 influences the development of cancer and cardiovascular disease. I will conduct the project in the membrane biology group of Prof. Matthias Hediger at the Institute of Biochemistry in Bern, Switzerland.
This fellowship will allow me to acquire all the skills and expertise necessary to become an independent researcher. Having a strong background in Basic Science and Medicine, I am convinced to be able to contribute significantly to the promotion of an original and sustainable ion transport research in Europe.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pharmacology and pharmacy drug discovery
- natural sciences chemical sciences inorganic chemistry alkali metals
- medical and health sciences basic medicine physiology pathophysiology
- medical and health sciences clinical medicine cardiology cardiovascular diseases
- medical and health sciences clinical medicine oncology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
BERN
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.