Objective
Antigen specific activation of B cells is mediated by the B cell receptor complex (BCR). Signal transduction of this receptor is regulated by the equilibrium of kinase and phosphatase activity. Syk protein tyrosine kinase and Src homology 2 domain containing protein tyrosine phosphatase 1 (SHP-1) has prominent role in the establishment of this equilibrium.
The aim of this proposal is to study the regulation of SHP-1 function. SHP-2 belongs to the same family of phosphatases, have high structural and sequence homology to SHP-1 but in contrast play mostly positive regulatory role in cell functions. The main goal of the project is to identify the modifications; molecular interactions that control the activity and intracellular localization of these enzymes and to compare the mechanisms of their regulations.
The major sequence differences of these phosphatases are in their C-terminal tail and probably this part is responsible for their functional divergence. This part of the protein contains two tyrosine and one-serine residues in SHP-1 and also two tyrosines and two serines in SHP-2. These amino acids are phosphorylation targets. My goal is to identify the kinase/kinases that phosphorylates them and other interacting molecules that can bind them and may regulate the phosphatases. For this purpose I will use the S2 Schneider cell reconstitution method that was developed in the host laboratory.
By the inducible and transient co-transfection of signalling molecules this method allows the rebuilding of signalling pathways in an evolutionary distant system and also to exclude the redundancy that is typical for molecules in mammalian signalling networks. SHP1 deficiency can lead to autoimmunity therefore revealing the molecular mechanisms of the regulation of this phosphata se may help to understand the development of certain autoimmune diseases.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences electrochemistry electrolysis
- engineering and technology environmental engineering waste management waste treatment processes remanufacturing
- medical and health sciences basic medicine immunology autoimmune diseases
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.