Final Activity Report Summary - MEMPROT (Structural studies of membrane proteases.)
In this project, we have attempted to purify membrane proteins, and particularly membrane proteases, both from natural sources and from genetically modified expression cells. So far, we have been able to grow crystals of one membrane protein complex, the photosystem II from the tobacco plant. This work is described in the publication "Crystallisation of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotiana tabacum. Piano D, El Alaoui S, Korza HJ, Filipek R, Sabala I, Haniewicz P, Buechel C, De Sanctis D, Bochtler M., Photosynth Res. 2010 Nov 10, epub ahead of print", which is attached to this report. Crystallisation of the other membrane embedded proteases that we have been able to express is still on-going.