Objective
The goal of this project is to develop qualitative and quantitative screening methods to identify and follow the fate of Se-species in Se-enriched functional foods. The interest behind the choice of selenium is driven by the state-of-the-art theoretical knowledge on its direct and indirect effects on cancer prevention that strongly depend on the given Se-species acting in in-vitro studies or in human metabolism.
Unfortunately, the actual distribution of Se-species in real world matrices is hardly known. The analysis of Se-containing or Se-enriched functional foods, a well-promoted part of innovative food products gives the possibility to analyse samples with challenging matrices and to characterise the Se-species that are putatively active in cancer prevention.
Among its main objectives the project includes the
- unambiguous identification of Se-species and their possible degradation products by the means of time-of-flight and hybrid triple quadrupole/linear ion trap mass spectrometry through accurate mass measurements and tandem MSn fragmentation studies,
- quantification of the Se-species by inductively coupled plasma mass spectrometer (ICP-MS) equipped with a collision cell,
- the synthesis of new Se-peptides for the quality control of sample preparation steps,
- time-resolved qualitative and quantitative monitoring of Se-species in selected functional food samples, ending up in the form of a database on the Se-species monitored.
An elegant method to detect and possibly identify all Se-species extracted from the samples will be the subsequent hyphenation of capillary flow HPLC separation based on reversed phase (RP) or hydrophilic interaction (HILIC) mechanisms to ICP-MS and to organic MS instrumentation.
The project will provide the possibility f or me to fully disseminate the experience gained during the initial fellowship in the field of organic mass spectrometry to the PhD and undergraduate students of the Host.
Fields of science
Not validated
Not validated
Call for proposal
FP6-2004-MOBILITY-11
See other projects for this call
Funding Scheme
ERG - Marie Curie actions-European Re-integration GrantsCoordinator
BUDAPEST
Hungary