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Content archived on 2024-06-16

Structural & dynamical characterization of the pro-metastatic enzyme heparanase for the designing of novel inhibitors

Objective

The focus of the project is the understanding of the biological functions and the structural characterization of heparanase. Heparanase is an endo-b-D-glucosidase capable of specifically degrading heparan sulfate, one of the extracellular matrix (ECM) components. This activity is associated with the metastatic potential of tumor cells, because these cells require the ability to degrade the ECM. During the last decade, NMR spectroscopy has become a leading technique to study biomolecules. Nowadays, its versatility allows the three-dimensional structure determination of biological macromolecules, the characterization of protein dynamics, kinetic and thermodynamic information on protein folding, as well as on molecular interactions.

This last issue has revealed NMR as an important tool for the Drug Discovery. The major limitation of NMR arises from the increase of transverse relaxation rates which prevents the investigation of molecules with large molecular weights. Heparanase is initially expressed as a 543 aminoacid polypeptide (65 kDa). The study of a protein of this size represents a challenge for NMR methodologies currently available.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP6-2004-MOBILITY-11
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Funding Scheme

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ERG - Marie Curie actions-European Re-integration Grants

Coordinator

CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE
EU contribution
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Address
Avenida Autopista del Saler 16-3 (junto al Oceanográfico)
VALENCIA
Spain

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