Objective
Dendritic cells in peripheral immune organs are quiescent sentinels that continuously survey their surroundings by fluid phase uptake and endocytosis. Dendritic cells become activated when pathogen-derived fragments are detected. Engulfed material is now processed into peptides and lipids for incorporation into peptide/MHC or lipid/CD1 complexes, for display to MHC- and CD1-restricted T cells respectively. The presentation of antigen to T cells is regarded the initiation of immune responses. Much of what we know about the intracellular antigen processing and loading has been obtained from biochemical and cell biological analysis of B cell lines, and to a more limited extent, of cultured dendritic cells.
New methodology now makes it possible to visualize the uptake of fluorescent antigen by live Class II MHC-positive dendritic cells in situ without the need for fixation or staining, by analysis of dendritic cells in the epidermis of mice that carry fusion proteins composed of Class II MHC molecules and green fluorescent protein. We will study the protein and lipid antigen-presentation pathways in skin-derived dendritic cells. We propose to study the importance of antigen entry into specialized endosomal compartments in prototypic immature dendritic cells in situ, by analysis of Langerhans cells in epidermis.
The loading of lipid antigens in late endosomal compartments in primary cells has not been explored to date, due to the unavailability of suitable mouse models. We have very recently succeeded in the generation of novel fluorescent knock-in mice in which we shall study the intracellular trafficking routes of membrane protein CD1d.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine immunology
- natural sciences biological sciences biochemistry biomolecules lipids
- medical and health sciences basic medicine pathology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
UTRECHT
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.