Objective
Phytoplasmas are insect-transmitted intracellular bacterial pathogens that infect plants in domesticated (crops) and natural habitats worldwide. Typically, they induce shoot proliferation and differentiation of floral parts into leafy structures, suggesting interference with basic cellular and developmental pathways.
The applicant has led a consortium that sequenced the 724 kbp genome of aster yellows phytoplasma strain witches¿ broom (AY-WB), and is involved in two further phytoplasma sequencing programmes. Analysis of the AY-WB sequence identified 58 candidate virulence proteins, of which 8 have nuclear localization signals (NLSs). Two of these proteins (A11 and A30) target nuclei in plant cells.
The objective of this proposal is to functionally characterize those candidate virulence proteins that target plant nuclei, with the hypothesis that they could change gene expression profiles through interactions with transcription factors and regulatory sequences. This work takes advantage of the broad host range of AY-WB that includes Nicotiana benthamiana, tomato, and Arabidopsis.
The specific aims of the proposal are:
- to determine the localization of all AY-WB NLS-containing proteins in plants;
- to conduct expression-profiling experiments on plants exposed to A11 and A30;
- to assess whether A11 and A30 bind regulatory sequences and transcription factors and how this may affect host susceptibility.
Relative to other work on bacterial virulence in plants, the work is distinctive in focusing on virulence factors that target nuclei and on intracellular bacterial pathogens. Finally, this project will transfer research experience and knowledge obtained during a seven-year career in the USA to the John Innes Centre, Norwich, UK.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture
- natural sciences biological sciences zoology entomology
- natural sciences earth and related environmental sciences atmospheric sciences climatology climatic changes
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NORWICH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.