Objective
The human genome has only about 24,000 protein-coding genes, considerably less than it was previously suspected. Much of the complexity of higher organisms can be attributed to alternative splicing (AS), and more than 70% of human genes are alternatively spliced. However, the number of functional proteins in humans, mice or Drosophila is still entirely uncertain as the putative proteins are identified from nucleic acid sequence information.
As there are several databases of AS that contain putative alternative splices assembled from the available EST- and cDNA-based evidence, we are planning to screen these AS isoforms as to whether they have full-length domains and if they can be folded into a stable 3D structure. In the first step we will filter the isoforms with our domain database, which contain only domains, derived from Pfam, that are always full-length (90% of the available Pfam families). In the next step we will mine the annotations of those human Swissprot proteins that have both AS and 3D-structure information, supplemented with orthologous proteins from other organisms and tissue-specific information to create a test set where we can determine with a high level of confidence if the observed splice variants exist in nature or not. We are going to use this test set to generate probabilistic models where we can calculate the probability if a certain AS isoform can exist as a stable isoform. The test set will be also used in evaluating the AS isoform models by structural calculations focusing on the newly discovered connection between alternative splicing and protein disorder. Some calculations will be performed at the High Performance Computing Centre of the University of Szeged in collaboration with theoretical physicists. The added value of this multidisciplinary approach will be hopefully both a better understanding of alternative splicing and the refining of the force fields and local effects used in the molecular modeling of disordered proteins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences computer and information sciences databases
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering computer hardware supercomputers
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-12
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
BUDAPEST
Hungary
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.