Objective
To establish a reliable methodology for the production of hybrid varieties in ryegrass (Lolium perenne) and white clover (Trifolium repens) by applying cytoplasmic male sterility and self-incompatibility respectively.
To investigate the nature of mtDNA in male fertile and male sterile stock and study the origin of this induced male sterility by molecular techniques.
To investigate the use of in-vitro pollination and seed set as a means of by-passing the self-incompatibility mechanism of white clover and also to determine if in-vitro methods could be used effectively to produce intergeneric crosses between T. repens and other forage legumes.
A study was made of the synthesis of hybrid cultivars of ryegrass (Lolium perenne) and white clover (Trifolium repens). In ryegrass the hybrid methodology is based on cytoplasmic male sterility. Some 14 maintainer lines have been produced from over 1000 genotypes and the programme is prepared to move forward with reciprocal crosses using nuclear gene restoration. In white clover a wide range of partially inbred lines homozygous for the self incompatibility locus have been obtained and test crossed. In vitro procedures for improved self pollination and seed set were developed; these methods can also be applied to interspecific hybridization. Methods for direct ovarian pollination of ovules were also developed.
The methods give promise for successful application is breeding programmes of ryegrass and white clover. Their applicability to other crops deserves increased attention.
- Glasshouse : Pair crossing, screening of progenies, reciprocal test crosses, pollen staining.
- Field : Field plot evaluation and progeny testing, isolation of breeding populations.
- Lab : Pollen examination, fluorescent microscopy, tissue culture, in-vitro pollination. DNA extraction, purification and analysis of restriction enzyme products.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy
- agricultural sciences agriculture, forestry, and fisheries agriculture industrial crops fodder
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds legumes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
4 Dublin
Ireland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.