Objective
The mesoderm of the amphibian embryo is induced by signals derived from the vegetal hemisphere of the embryo, which act on overlying equatorial cells. This proposal investigates the role and mode olfaction of the mesoderm-inducing factor active; recent experiments using antisepses morpholinooligonucleotides have shown that active is required, in a dose-dependent fashion, for normal mesoderm formation. We shall first use DNA microarray to identify genes that are down regulated in embryos in whichactivin signalling is reduced or abolished. The expression patterns of genes identified in this way will be investigated by in site hybridisation and their responses to different levels of active signalling will be studied. The 5' regulatory regions of active target genes will be analysed to ask if they share common motifs or transcription factor binding sites. The second series of experiments investigates the range of active signalling; experiments in whichactivin is miss-expressed in ectodermic tissue indicate that active can exert its effects over many cell diameters. However, it is important to know the range of endogenous active signalling in the intact embryo and whether this varies in different regions of the embryo. This will be studied by making clones of cells in which active translation is inhibited and asking to what extent neighbouring cells can rescue' target gene expression in those cells. The third question concerns post-transcriptional regulation of active; the effects of active are exerted predominantly in the vegetal hemisphere and marginal zone of the embryo, but active B mRNA is expressed in the animal hemisphere as well as in vegetal cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.