Objective
Deep-sea hydrothermal vents are microbiologically well characterised, light-independent ecosystems where primary production is completely based on chemo autotrophy. In contrast, shallow water hydrothermal vents (SWHVs) have been largely ignored by microbial ecologist although these sulphide-rich vents are exciting model systems for the co-occurrence of chemoautotrophic and phototropic microbial communities. This project intends to provide an in depth picture on the biodiversity and spatial distribution of the microbial communities involved in biogeochemical cycling of sulphur at a tropical SWHV at the west coast of Dominica. For the first time, state-of-the- art, cultivation-independent techniques for microbial community analyses will be applied to such systems. The obtained data will be combined with physicochemical analyses of the SWHV fluids and the surrounding sediment. A recently developed 16S reran-based oligonucleotide micro array for the detection of all sulphate- reducing prokaryotes (Saps) will be extended to also cover all yet known oxidizing-oxidizing prokaryotes (SOPs). This DNA micro array will then be used to rapidly screen for the richness of these ecologically important sulphur- cycling microbial guilds at the SWHV. In addition, yet uncultured Saps will be identified by phylogenetic surveys of dissimulator (bi)sulphite reduces genes (drab) retrieved from the SWHV samples. Based on these data, the spatial distribution and abundance of Saps and SOPs will be quantitatively analysed by fluorescence in site hybridisation in combination with high-end confocal laser scanning microscopy and digital image analysis. These investigations on free living and benthos prokaryotes will be complemented by including prokaryotes occurring as simians of animals at the vent site. The phylogeny, abundance and distribution of the microbial simians will be analysed by 16S reran sequence retrieval and quantitative FISH.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biological morphology comparative morphology
- natural sciences biological sciences microbiology microbiomes
- natural sciences biological sciences ecology ecosystems
- natural sciences physical sciences optics microscopy confocal microscopy
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WIEN
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.