Objective In the pathways of gene expression, by which information is transferred from DNA to end-up with a functional protein, the first regulated and in most cases rate-limiting step is the process of transcription. Despite the detailed picture that is emerging about the composition of the RNA polymerase-ll machinery, a great number of conceptual as well as mechanistic questions are still needed to be resolved before we can attain a complete picture of the principles employed by living organisms to control this process. One of the main gaps in our knowledge is the limited insight we have on transcription regulation in the nuclear environment. The dynamic association of RNA polymerase-ll with general transcription factor complexes within the nucleus, the trafficking of the components between nuclear structures, the forces governing complex-complex interactions and the pacemaker of their intrinsic dynamics at the DNA-protein surfaces of the genes is poorly understood.The general goal of the network is to obtain a comprehensive knowledge on the mechanism of regulation of model genes during cell differentiation, cell proliferation and signal transduction. The network will undertake concerted efforts to develop and apply fluorescent imaging techniques, coupled with genetic, proteomic and conventional molecular and cell biology approaches to study the molecular characteristics and functions of individual multiprotein complexes and their dynamic interplay in the context of unique chromatin structures in living cells.Successful execution of the project is expected to result in:i. New knowledge on the in-situ dynamics of RNA polymerase-ll machinery,ii. New knowledge on the molecular mechanism of transcription regulation that leads to pathway-specific gene expression programs,iii. Development of new technologies and research tools. Fields of science natural sciencesmathematicspure mathematicstopologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencescell biologynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticsRNA Keywords II RNA polymerase RNA polymerase-II gene expression in situ dynamics transcription complexes Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Call for proposal FP6-2002-LIFESCIHEALTH See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS Address Vassilika vouton Heraklion Greece See on map EU contribution € 0,00 Participants (6) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITY OF SZEGED Hungary EU contribution € 0,00 Address Dugonics ter 13 Szeged See on map CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Rue michel ange 3 Paris See on map GSF - FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT GMBH Germany EU contribution € 0,00 Address Ingolstaedter landstrasse 1 Neuherberg See on map NATIONAL HELLENIC RESEARCH FOUNDATION Greece EU contribution € 0,00 Address Vassileos constantinou avenue 48 Athens See on map UNIVERSITAIR MEDISCH CENTRUM UTRECHT Netherlands EU contribution € 0,00 Address Heidelberglaan 100 Utrecht See on map CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE France EU contribution € 0,00 Address Rue laurent fries 1 - igbmc Illkirch graffenstaden See on map
