Objective
Increasing evidence indicates that eukaryotic cells make use of specific nutrient signalling pathways for control of growth and growth-related properties. In the yeast Saccharomyces cerevisiae, the protein kinase Sch9 plays an important role in nutrient-induced signal transduction and growth control, but the precise mechanisms involved are not well understood. Sequence similarity suggests that protein kinase Sch9 represents the yeast homologue of mammalian protein kinase B (PKB). A role of Sch9 in nutri ent-induced signalling is indicated by the observation that deletion of the SCH9 gene deregulates fermentable growth medium (FGM) signalling. The fermentable growth medium (FGM)-induced pathway maintains activation of protein kinase A targets during gro wth on a fermentable carbon source like glucose. In this project several approaches will be taken to identify new components of the Sch9 nutrient signalling pathway. First, downstream components of Sch9 will be identified as multi-copy suppressor genes o f the growth defect caused by deletion of Sch9 in a specific genetic background. Second, upstream activators of Sch9 will be identified by transposen mutagenesis using a specific genetic background in which inactivation of the upstream components is leth al and can be rescued by induction of Sch9 overexpression. The relationship between the new components identified and other components closely connected to Sch9, such as PKA, Rim15, Yak1 and the two Pkh proteins will be established using epistasis analy sis. Execution of this project will allow advanced training in yeast molecular genetics, recombinant-DNA technology, bioinformatics and determination of a variety of biochemical parameters. In addition, the applicant will be able to develop her skills in research supervision and her potential for independent scientific research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ZWIJNAARDE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.