Objective
Belgian Texel sheep are characterized by an inherited muscular hypertrophy, controlled by a major locus mapped to the pericentric region of sheep chromosome 2. The purpose of this project is to identify the corresponding gene and mutation. Knowledge of t his gene will allow for more effective marker assisted selection, will open possibilities for targeted development of drugs that could be useful in the treatment of muscle wasting, and will identify a potential target for engineering using transgenic te chniques. Identification of the causal gene and mutation will be achieved by means of positional cloning. To that end we will first improve the genetic and comparative map of the region. Bovine BAC libraries will be screened with available microsatellit es in the region of interest. Fingerprinting contigs (FPC) including the positive BACs will be identified by searching the ICE database compiled by the Genome Sequence Centre, Vancouver, Canada (http://www.bcgsc.bc.ca/(opens in new window)). Novel microsatellites will be iso lated from the corresponding BACs. BAG ends will be sequenced and BLAST searches will be performed against the human genomic sequences to identify orthologous anchorpoints. BAC ends corresponding to the extremities of FPC will be used as probes to scree n the BAG libraries, thereby completing the contig of the region by \\\"FPC walking\\\". Genetic fine-mapping will be accomplished with the new markers by exploiting linkage in the available F2 pedigree, and by exploiting linkage disequilibrium analysis within the Belgian Texel population (all double-muscled Belgian Texel are expected to share a common haplotype spanning the causal gene). We will identify (b)ovine cDNAs orthologous to the genes mapping to the corresponding human region using the EGO d atabase available from TIGR (http://www.tigr.org/(opens in new window)) and assemble spotted cDNA microarrays including all these positional candidates.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LIEGE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.