Objective
Streptomycetes are mycelial soil bacteria that undergo a complex developmental life cycle on solid media,which is accompanied by production of secondary metabolites, e.g.antibiotics. Streptomyces coelicolor is the model Streptomycete and its 8.7Mb genome h as an unusually high number (965) of putative regulatory genes including 65 sigma factors as well as putative gene clusters involved in anaerobic metabolism. The latter was unexpected as Streptomyces are considered to be obligate aerobes. Recently I have s hown that morphological differentiation of S. coelicolor also occurs in standing liquid culture (SLC). After a period of submerged growth, hyphae migrate to the air interface, where they become fixed by a rigid reflecting film. The resulting colonies form sporulating aerial hyphae. In contrast, shaken liquid cultures of S. coelicolor do not differentiate. In addition, SLCs rapidly become anoxic only 1 to 2mm below the surface, implying the existence of metabolic pathways supporting anaerobic growth or enabl ing the organism to survive long periods of low exygen conditions. The proposed project aims to understand growth, stress responses and developmental pathways at the molecular level in S. coelicolor SLCs. This will be done by identifying differentially exp ressed genes in liquid and solid media using DNA microarrays and proteomics. The roles of the identified genes in morphological differentiation, stress and anaerobic/microaerophilic growth will be investigated using the rapid PCR-di rected gene-disrupti on method developed at the host institute, and through biochemical characterization of selected proteins. The project will be carried out at the John Innes Centre (JIC) under the supervision of Dr. M. Buttner and Dr. G. Sawers. The JIC offers a stimulating research environment as well as world-leading expertise in the analysis of S. coelicolor functional genomics, morpholoogical differentiation, stress responses, and antibiotic biosynthesis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences genetics genomes
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
NORWICH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.