Objective
Virulence of the plant pathogenic bacteria Erwinia carotovora subsp. carotovora (Ecc) depends on the production and secretion of the extracellular plant cell-wall degrading enzymes, which synthesis is controlled by a complex regulatory system. N-acyl homos erine lactones (HSLs) are involved in induction of the cell wall degrading enzymes synthesis. The HSLs are common signaling molecule of Gram-negative bacteria and play an important role in cell to cell communication between bacterial cell as well as in the interaction between bacteria and higher organisms. Synthesis of HSLs is dependent on the population density and a phenomenon known as "quorum-sensing". Soil bacteria have been identified to be able to degrade HSLs and thus interfere with quorum-sensing. S o far two enzymatic activities have been characterised: an acyl-HSL-lactonase from Bacillus sp. and Agrobacterium tumefaciens and an HSL-acylase from Variovorax paradoxus. Potentially, these enzymes offer a new tool to interfere with virulence factors of ( plant) pathogenic bacteria, which synthesis is regulated by HSLs. The aim of this project will be to characterise an HSL degrading factor produced by bacterial soil species never described before (Ochrobactrum sp. or Delftia). In the initial studies the so il isolates were tested for their capacity to degrade HSL and to inhibit Ecc pathogenicity. One of the pre-selected isolates will be selected for further analysis. Genes involved in HSL-degradation/inactivation will be cloned, and the putative protein will be expressed and purified. Transposon mutagenesis or a cosmid library of a genomic DNA of selected isolate will be used as the potential strategies for a characterisation of the gene encoding HSL-degrading enzyme. The DNA sequence of the putative gene wil l be analysed and the potential protein will be purified. The effect of HSL-degradation of the purified protein will be tested. For that, a Green Fluorescence Protein #
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-11
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
GDANSK
Poland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.