Objective
Hormones are implicated in almost every aspect of plant growth. Selective proteindegradation by the ubiquitin-proteasome pathway has emerged as a powerful regulatorymechanism in hormone signal transduction. A clear role for ubiquitin-mediated proteolysis o fthe AUX/IAA transcriptional repressers is already demonstrated. Recent studies providedpreliminary evidence for the involvement of ubiquitin-mediated proteolysis in the signalingpathways of other classical plant hormones like cytokinins, gibberellins, eth ylene and ABA.Genetic approaches have generated a number of mutants in ABA biosynthesis or ABAaction. One of the cloned genes encodes ABA-INSENSITIVE3 (ABI3), a positive regulator ofthe late maturation programme in embryogenesis, and simultaneously as a ne gativeregulator of germination programmes. Four ABIS-interacting proteins (AlPs) were identifiedin a yeast 2-hybrid screen. One of these encoded a protein (AIP2) with a C3HC4 RING fingermotif at its C-terminus. As RING-finger proteins are likely to functio n as E3 ligases, anessential component of the ubiquitination machinery, it is reasonable to hypothesize thatAIP2 attenuates ABA signals by down regulating ABI3. In this proposal this hypothesis willbe tested. The interaction between AIP2 and ABI3 will be v erified and other AIP2-interactingproteins will be identified using yeast two-hybrid technology. Their interacting domains willbe identified. To investigate if AIP2 has E3 activity and specifically targets ABI3 as asubstrate, recombinant AIP2 will be used as a source of E3 in an in vitro ubiquitinationexperiment with ABI3 as a substrate. Transgenic plants overexpressing AIP2 will beproduced and analysed together with mutants in AIP2. The expression profiles of AIP2 willbe analysed using transgenic plants ca rrying a fusion protein of the AIP2 promotor and theß-glucuronidase or GFP-gene. Using pull-down experiments, in vitro and in vivo #
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences biological sciences zoology mammalogy
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-6
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
OIF - Marie Curie actions-Outgoing International Fellowships
Coordinator
ZWIJNAARDE
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.