Objective
All living cells are surrounded by a membrane which provides a barrier between the contents of the cell and its environment. Various crucial processes in cells, such as cell/organelle division, or endo-/exo-cytosis, require the fusion of membranes. In e ukaryotes, various proteins have been identified that are involved in different membrane fusion events. In bacteria, membrane fusion occurs during cell division and sporulation. Recently, the first bacterial protein has been identified that is involved i n membrane fusion. This protein, SpolllE, is necessary for the membrane fusion reaction that occurs at a late stage in sporulation in Bacillus subtilis. SpolllE is a member of the SpolllE/FtsK family of proteins which is widely conserved in bacteria. Sp olllE/FtsK are involved in cell division and sporulation, and localise at the leading edge of the invaginating membrane. The proteins consist of an N-terminal region containing four transmembrane regions, a linker region, and a C-terminal domain that me diates energy-dependent DNA transport. During cell division, SpolllE/FtsK are critical for correct chromosome partitioning, whereas in sporulating bacteria, SpolllE transports DNA from the mother-cell into the prespore. This project focuses on the role of the N-terminal membrane segment of SpolllE/FtsK in membrane fusion and DNA transport. The following questions will be addressed: 1) Do the transmembrane regions of SpolllE/FtsK oligomerise to form a DNA pore? 2) Do the transmembrane regions of Spolll E/FtsK mediate membrane fusion? 3) What is the energy requirement of DNA transport across a biological membrane? Full-length SpolllE/FtsK and truncates containing only the transmembrane region will be purified and reconstituted in artificial liposome sys tems. These systems allow a detailed characterisation of both the membrane fusion and DNA transport reactions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics chromosomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-11
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.