Final Activity Report Summary - EPIARABIDOPSIS (Characterization of the epigenetic nature of a gametophytic lethal mutation in Arabidopsis rhaliana)
We performed the morphological and genetical characterisation of both AtSWI3B mutants, showing that the AtSWI3B disruption originates by itself an embryo lethal phenotype, and it is associated to a gametophyte defect caused by the hypothetical epigenetic modification of other loci. Partial positional mapping of the gametophytic lethality and crossings with qrt1 mutant suggest the involvement of two independent loci on the phenotype.
Different T-DNA collections have been searched for mutants in other Swi / Snf coding genes, and the effect of these mutations will be described. Finally, plants expressing Swi3-like and Bsh (Snf5-like) proteins fused to an epitope will allow the tracking of these proteins, and the purification and co-immunoprecipitation of Arabidopsis Swi / Snf complexes.
We also isolated the SNF5-like gene from pea (Pisum sativum) and described its expression dependent on ABA and desiccation.