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Development of new methodologies for low abundance proteomics: application to cystic fibrosis

Objective

The objective of this proposal is to identify new low and medium abundance proteins involved in the pathophysiology of cystic fibrosis (CF), proteins that may constitute new diagnostic and prognostic biomarkers and be helpful in the development of new dr ugs for CF. To achieve this we will develop tools and standardize protocols for new approaches to study proteomics and facilitate generation of new knowledge in functional genomics. High performance techniques will be used and/or developed: Radio-isot ope and classical detection methods for differential display of proteins including their splicing variants and post-translational isoforms, i.e. the supersensitive analysis of 2D electrophoresis gels by a radio-isotopic technique (ProteoTope), Hig hly sensitive chromatographic methods for lipid analysis associated with different proteomic approaches for proteome-lipidome interactions, Surface plasmon resonance (Biacore®) for detection of interacting proteins, Protein sequence determination by mass spectrometric techniques (MS) using new, high sensitivity modalities including, Surface-Enhanced-Laser-Desorption-Laser/Ionization Time-of-Flight Mass Spectrometry (MS), FTICR-MS , multidimen- sional liquid chromatography -MS, Innovative bio-i nformatic tools. Our methodological goals are: To apply an integrated proteomic system for the detection of low and medium abundance proteins to different biological models of CF (serum, nasal ciliated cells, neutrophils, cell lines), To improve th e sensitivity of MS for the peptide sequences of differentially displayed proteins, To develop the MS methodology for analysis of protein without 2D gel protein separation To develop new tools for protein-, DNA-, and lipid- protein analysis. Our proje ct main goals are: To develop and apply highly sensitive methods for low abundance protein identification by MS which will be useful for the entire proteomic community,

Call for proposal

FP6-2003-LIFESCIHEALTH-I
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Funding Scheme

NoE - Network of Excellence

Coordinator

INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE
Address
101 Rue De Tolbiac
Paris
France

Participants (11)

INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS, POLISH ACADEMY OF SCIENCES
Poland
Address
Pawinskiego 5A
Warsaw
UPPSALA UNIVERSITY
Sweden
Address
Sankt Olofsgatan 10B
Uppsala
UNIVERSITY COLLEGE LONDON
United Kingdom
Address
Gower Street
London
UNIVERSITY OF WALES, CARDIFF
United Kingdom
Address
7Th Floor, 30-36 Newport Road
Cardiff
INSTYTUT MATKI I DZIECKA
Poland
Address
Kasprzaka 17A
Warsaw
KAROLINSKA INSTITUTET
Sweden
Address
Nobels Vägs 5
Stockholm
INSERM-TRANSFERT SA
France
Address
101 Rue De Tolbiac
Paris
PROTEOSYS AG
Germany
Address
Carl-zeiss-str. 51
Mainz
UNIVERSITAET ULM, MEDIZINISCHE FAKULTAET
Germany
Address
Albert-einstein-allee 29
Ulm
ASSISTANCE PUBLIQUE-HÔPITAUX DE PARIS,
France
Address
Avenue Victoria, 3
Paris
FUNDAÇÃO DA FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
Portugal
Address
Campo Grande - Edifício C7
Lisboa