Objective
The objective of this proposal is to identify new low and medium abundance proteins involved in the pathophysiology of cystic fibrosis (CF), proteins that may constitute new diagnostic and prognostic biomarkers and be helpful in the development of new dr ugs for CF. To achieve this we will develop tools and standardize protocols for new approaches to study proteomics and facilitate generation of new knowledge in functional genomics. High performance techniques will be used and/or developed: Radio-isot ope and classical detection methods for differential display of proteins including their splicing variants and post-translational isoforms, i.e. the supersensitive analysis of 2D electrophoresis gels by a radio-isotopic technique (ProteoTope), Hig hly sensitive chromatographic methods for lipid analysis associated with different proteomic approaches for proteome-lipidome interactions, Surface plasmon resonance (Biacore®) for detection of interacting proteins, Protein sequence determination by mass spectrometric techniques (MS) using new, high sensitivity modalities including, Surface-Enhanced-Laser-Desorption-Laser/Ionization Time-of-Flight Mass Spectrometry (MS), FTICR-MS , multidimen- sional liquid chromatography -MS, Innovative bio-i nformatic tools. Our methodological goals are: To apply an integrated proteomic system for the detection of low and medium abundance proteins to different biological models of CF (serum, nasal ciliated cells, neutrophils, cell lines), To improve th e sensitivity of MS for the peptide sequences of differentially displayed proteins, To develop the MS methodology for analysis of protein without 2D gel protein separation To develop new tools for protein-, DNA-, and lipid- protein analysis. Our proje ct main goals are: To develop and apply highly sensitive methods for low abundance protein identification by MS which will be useful for the entire proteomic community,
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics quasiparticles
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2003-LIFESCIHEALTH-I
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.