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Methods for the concentration and detection of adenoviruses and noroviruses in European bathing waters with reference to the revision of the bathing water directive 76/160/EEC

Objective

The Project will provide a procedure for analysis of EU bathing waters for noroviruses and adenoviruses by validated comparisons of methods for processing water samples to achieve the best virus recovery consistent with cost and feasibility of use in routi ne monitoring laboratories. Objectives are (a) compare methods fornorovirus and adenovirus detection in recreational waters (b) derive a combination of concentration and detection techniques to provide a reproducible system of testing bathing waters for th e target viruses (c) furnish scientific evidence to provide support for norovirus and adenovirus testing of environmental samples in respect of their role as the appropriate viral indicator of faecal pollution (c) prepare the technology for Accession State s as part of the development of their environmental and social programmes (d) share technology between laboratories to achieve wider competence in the virological analysis of environmental materials. Detection by PCR and cell culture together with the conc entration procedure will provide a combined technique. PCR products will be sequenced and data analysed to derive strain and serotype information.The work addresses the research objectives of SSP 8.1 task 1.5 directly through relevance to the revision of t he Bathing Water Directive. Inter-Laboratory comparisons and a large field based surveillance Phase are integrated to ensure that the new combined method will have immediate applicability in EU bathing water monitoring. It will be done by 16 Participant La boratories in a unified approach to derive a harmonised combined method to provide credibility for future monitoring regimes give the potential to place a virus parameter on a footing equal to the bacterial indicators. Inclusion of Laboratories representat ive of the Accession States will ensure rapid dissemination to enhance the monitoring of their bathing waters and thus sustain the development of their own tourism and that of the European tourism worldwide.

Call for proposal

FP6-2003-SSP-3
See other projects for this call

Funding Scheme

STIP - Specific Targeted Innovation Project

Coordinator

UNIVERSITY COLLEGE OF WALES ABERYSTWYTH
Address
Old College, King Street
Aberystwyth
United Kingdom

Participants (15)

CENTRAL SCIENCE LABORATORY
United Kingdom
Address
Nobel House 17 Smith Square
London
RIJKSINSTITUUT VOOR VOLKSGEZONDHEID EN MILIEU
Netherlands
Address
Antonie Van Leeuwenhoeklaan 9
Bilthoven
UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA
Italy
Address
Via Orazio Raimondo 18
Rome
ENVIRONMENT AGENCY
United Kingdom
Address
Rio House, Waterside Drive, Aztec Way, Almondsbury
Bristol
UNIVERSITAT DE BARCELONA
Spain
Address
Gran Via De Les Corts Catalanes, 585 (Vicerectorat De Política Científica (Oper)
Barcelona
UMWELTBUNDESAMT (GERMAN ENVIRONMENTAL AGENCY)
Germany
Address
Bismarckplatz 1
Berlin
PANSTWOWY INSTYTUT WETERYNARYJNY - PANSTWOWY INSTYTUT BADAWCZY W PULAWACH
Poland
Address
Al. Partyzantow 57
Pulawy
ISTITUTO SUPERIORE DI SANITA
Italy
Address
Viale Regina Elena 299
Roma
FACULDADE DE FARMACIA DA UNIVERSIDADE DO PORTO
Portugal
Address
Rua Anibal Cunha, 164
Porto
STATE GENERAL LABORATORY
Cyprus
Address
44, Kimonos Street
Nicosia
HEALTH PROTECTION AGENCY
United Kingdom
Address
61 Colindale Avenue
London
BAYERISCHES LANDESAMT FÜR GESUNDHEIT UND LEBENSMITTELSICHERHEIT
Germany
Address
Eggenreuther Weg 43
Erlangen
LANDESGESUNDHEITSAMT BADEN-WÜRTTEMBERG
Germany
Address
Wiederholdstr. 15
Stuttgart
UNIVERSITÀ DI PISA
Italy
Address
Lungarno Pacinotti, 43
Pisa
UNIVERSITÉ HENRI POINCARÉ - NANCY
France
Address
24-30 Rue Lionnois
Nancy